DKN-01 binds and removes free DKK1 from the TME:
Reduces cell proliferation
Blocks signaling through CKAP4 and PI3K to downregulate Akt
B-catenin dependent Wnt signaling reprograms MDSCs and reduces immunosuppressive activity
Reduces blood vessel formation, upregulates key cytokines, IFNy, IL-15 and IL-33
Upregulates NK cell ligands on tumor, production of Granzyme B by activated NK cells
DKN-01 + anti-PD1
DKN-01 stimulates innate
of NK cells
Upregulates PD-L1 (the target for PD-1 antibodies)
CD8 T cell adaptive immunity:
Enhances tumor microenvironment for immune cells
to target and clear
CD8 T cells
DKK1 expression determined using RNAscope
Chromogenic in situ
The biopsy sample is stained to identify DKK1 mRNA Pathologist determines histology score (H-Score), measuring DKK1 expression rather than protein itself.
Each red dot is an individual mRNA for DKK1.
Amount of cells and intensity of staining is converted to H-Score.